TYPES OF SERVICES
CONSULTATION
Our team of experienced scientists can guide you through the complexities of the drug discovery and development journey by providing a plan forward and strategic insights at different stages of your drug discovery journey.
ASSAY DEVELOPMENT, OPTIMISATION & VALIDATION
We help refine and redefine the accuracy of your assays following the core guiding principles of development, optimization, and validation. Our team designs assays to meet your specific needs, ensuring adherence to rigorous standards for reliability and reproducibility, crucial for the success of drug discovery initiatives.
DRUG SCREENING CAMPAIGNS
We use industry-leading technologies and drug libraries that match the latest in drug discovery trends to accelerate the identification of promising candidates through efficient high-throughput screening methods and expedite the path of development.
COMPOUND STAMPING
We provide compound stamping services for compounds supplied by our clients in ready-to-screen plate formats of your choice. We also offer a selection of high-quality commercial small molecules with adequate diversity, lead-likeness, and solubility profile.
Focused sets against a wide range of possible target proteins that are curated to increase the chances of finding hits from within a selection from the entire library to accelerate discovery.
Diversity sets for discovery biology and libraries of drugs that have cleared at least the 1st phase of clinical trials for target validation and drug repurposing studies.
Fragment libraries – smaller fragments of molecules as libraries to reduce size and complexity for more efficient chemical space sampling.
TYPES OF HIGH THROUGHPUT ASSAYS
CELL-BASED ASSAYS
A robust & sensitive cell-based assay is crucial for drug discovery campaigns to select desirable hits with on-target potencies. We offer an array of off-the-shelf and custom-designed assays to investigate and screen for compounds against your target of interest and disease biology.
Such as but limited not to:
Proliferation Assays: Quantify the cellular growth dynamics using various detection methods that provide essential insights into cell viability, replication rates, and response to various compounds with tropical toxicity to cell-population of interest, aiding in the identification of potential therapeutic targets.
Cell Signaling Assays: Determine the mechanism of action of the test compounds using our homogeneous signal transduction assays and monitor receptor-mediated modulation of pathways in response to the test compounds.
Reporter Assays: Investigate gene expression and regulation using custom-designed and optimized reporter assays for a detailed understanding of how compounds influence gene activity.
Ion Channel Assays: Screen, identify, and validate compounds against Ion Channels and GPCR targets using high-throughput homogeneous cell-based FLIPR assays. Gain crucial insights into potential drug candidates’ effects on cellular electrophysiology.
BIOCHEMICAL ASSAYS
Biochemical assays prove a rapid and efficient method to quantify & analyze the binding properties of the target protein and the effect of the compound on the target of interest. Over the years, we have optimized and miniaturized numerous biochemical assays for diverse target classes.
BIOPHYSICAL ASSAYS
Explore the physical properties of drug-target interactions. Confirming the affinity of test compounds to the protein of interest enables a deeper understanding of binding mechanisms and supports rational drug design.
HIGH CONTENT IMAGING ASSAYS
In contrast to the traditional single-end-point readout of activity, High Content Imaging uses automated microscope-based measurements of biological activity in 2D and 3D cultures following chemical or genomic perturbations, offering the ability to study multiple parameters simultaneously in complex biological systems. Typically, multiple features of the cell or organism are measured with one or more fluorescent dyes, hence the term High Content. The rich, multiparametric data on cellular morphology and function allows for a holistic evaluation of the effects of the agent.
Cell Painting: An information-rich, multidimensional profiling of biological activity using robust and highly specific dyes to paint multiple cellular organelles to predict a drug’s activity, toxicity, or mechanism of action to accelerate drug discovery. This offers an agnostic approach to discovering the unseen biological effects of chemical or genomic perturbations.
Biomarker Expression: Explore and quantify known biomolecules/targets within cells to enable a deeper understanding of disease pathways. High Content Imaging ensures accurate and multiparametric measurements to identify and validate potential biomarkers with great clarity.
Co-culture Modelling: In the context of late-stage cancer investigation, co-culture screening offers a great advantage in identifying therapeutics that selectively kill primary and/or metastatic tumor populations by mimicking complex cellular interactions in a controlled environment. This provides valuable insights into the behavior of potential drug candidates within physiologically relevant contexts.
3D Tumor Spheroid Modelling: Tumor spheroid models can be used to recapitulate complex tumor microenvironment, which is composed of cells in different proliferative and metabolic states for the development of new anticancer strategies using automated imaging and analysis methods to assess treatment effectiveness with higher predictive value.